This activity is open to DMU, Drake and Iowa State researchers only. There is no cost to attend and registration is not required. Lunch will be served.
 

Advanced Cre-lox: Generating Reporters, Inducible Mice, and Disease Models

Cre-lox technology is a widely used and sophisticated tool to create general knockouts, conditional knockouts, and reporter strains. Researchers worldwide utilize these models for a variety of applications, including specific disease research and visualization of mouse anatomy. 

• Identifying appropriate Cre-reporter strains to label specific cell populations or to perform mosaic and lineage tracing studies
• Breeding strategies and caveats to generate and use tamoxifen and doxycycline-inducible models
• Implementing Cre-lox technology to model various types of cancer, neurodegenerative disorders, and other human diseases.

Generating Mouse Models Using CRISPR/Cas Technology

Do you need to generate new mouse models within 3-4 months instead of 1-1.5 years? Revolutionary CRISPR/Cas technology enables rapid, economical model generation through precise genome editing. During this seminar, you will gain an understanding of the potential of CRISPR/Cas to accelerate your research. 

• Historical landscape of genetically engineered mouse models
• How CRISPR/Cas technology is used to generate mouse models
• Advantages and limitations of CRISPR/Cas technology
• Selection of founders and follow-up breeding

Achieving Reproducible Mouse Studies

Success in the drug development pipeline depends on success in basic and preclinical research. During this seminar, we will discuss unique experimental design considerations for mouse-based research. 

• Appropriate model and control selection
• Suitability of phenotypic readout
• Sample size planning